Synthesis and ribonuclease H related biochemical properties of α-anomeric 2′-deoxypyrimidine homooligomer and its phosphorothioate analog

RNase H mediated degradation of RNA on the β-DNA•RNA duplex was inhibited by the presence of extra 2′-deoxypyrimidine homooligomer. The effect was dependent on the nature of the nucleobase, glycoside bond configuration, and the internucleotide linkage of the extra homooligomer used. Thus, α-anomeric 2′-deoxycytidylate phosphorothioate exhibited the most potent inhibitory effect (>40%) on RNase H.