Novel non-nucleosidic phosphoramidites for oligonucleotide modification and labeling

A series of novel labeled phosphoramidites and CPG supports 1a-f with a new non-nucleosidic backbone based upon cyclohexyl-4-amino-1,1-dimethanol have been synthesized. These reagents have been used to label oligonucleotides with biotin and fluorescein at the 5′-, the 3′-, and internal positions.