Evidence for induced DNA bending by the yeast zinc cluster protein PUT3

We employed helical phasing analysis to study DNA conformation in the absence and presence of PUT3, a member of the Zn2Cys6 binuclear cluster family of transcription factors. Our data indicate that the PUT3 target site UAS10 does not bend appreciably in the absence of PUT3(1–100), but bends towards the major groove by approximately 46 degrees in the presence of PUT3(1–100).