Specific DNA binding peptide-derivatized solid support

The basic region (amino acids 226–252) of the basic region/leucine zipper protein GCN4 has been synthesized and covalently attached to a column matrix. This peptide dimer-derivatized column binds to the AP-1 (5′-TGACTCA-3′) and ATF/CREB (5′-TGACGTCA-3′) DNA sites, which are the target sites of native GCN4.