Display of fixed residues on variable cyclic scaffolds: A novel approach to peptide combinatorial library synthesis

A synthetic peptide library is described which displays three nominated residues in varied spacing around cyclic peptides. Diversity of ring size was achieved by the use of binary combinatorial synthesis, and the inclusion of hydrophilic spacer molecules. The order of residue occurrence and chirality was fully varied while duplication was avoided using a modified split-and-mix protocol.