TracermerTM signal generators: An arborescent approach to the incorporation of multiple chemiluminescent labels

The synthesis, conjugation, and chemiluminescent evaluation of zero, first, and second order acridinium-based TracermerTM signal generators are described. Members of this family of labels have potential use as tracers in diagnostic assays and are structurally similar to arborol dendrimers. TracermerTM-BSA conjugates showed up to a sixfold increase in light emission compared to the normal acridinium label.