Author/Authors :
Kyoji Furuta، نويسنده , , Takamitsu Hosoya، نويسنده , , Keiichiro Tomokiyo، نويسنده , , Sachio Okuda، نويسنده , , Akihiko Kuniyasu، نويسنده , , Hitoshi Nakayama، نويسنده , , Toshihisa Ishikawa، نويسنده , , Masaaki Suzuki، نويسنده ,
Abstract :
[35S]S-[5-(4-benzoylphenyl)pentyl]glutathione (GIF-0017) as a biochemical probe targeting the ATP-dependent organic anion transporters GS-X pumps was synthesized by the reaction of [35S]glutathione and excess 4-(5-bromo)pentylbenzophenone under alkaline conditions, with the radiochemical yield of 24–33% after HPLC purification. Photolysis of the mixture of [35S]GIF-0017 and plasma membrane vesicles prepared from the MRP1 cDNA-transfected LLC-PK1 cells resulted in radio-labeling of a 180-kDa membrane protein. Immunoprecipitation and western blotting using an anti-MRP1 monoclonal antibody confirmed that the [35S]GIF-0017-labeled protein was the MRP1/GS-X pump.
