9-[(2′S,3′S)-3′-formyl-2′,3′-dihydroxypropyl]adenine: A facile affinity-labeling probe of human hydrolase

Treatment of human recombinant (SAH) hydrolase with 9-[(2′S,3′S)-3′-formyl-2′,3′-dihydroxypropyl]adenine (FDHPA) caused irreversible inactivation in a time- and concentration-dependent manner (Ki = 8.8 μM, kinact = 0.09 min−1). FDHPA behaved as a facile affinity-labeling probe of SAH hydrolase. Abstract Treatment of human recombinant (SAH) hydrolase with 9-[(2′S,3′S)-3′-formyl-2′,3′-dihydroxypropyl]adenine (FDHPA) caused irreversible inactivation in a time- and concentration-dependent manner (Ki = 8.8 μM, kinact = 0.09 min−1). FDHPA behaved as a facile affinity-labeling probe of SAH hydrolase.