Author/Authors :
Kyle R. Gee، نويسنده , , Eric A. Archer، نويسنده , , Laura A. Lapham، نويسنده , , Mary E. Leonard، نويسنده , , Zhang-Lin Zhou، نويسنده , , John Bingham، نويسنده , , Zhenjun Diwu، نويسنده ,
Abstract :
Mono-halogenated derivatives of the calcium indicators fura-2 and indo-1 were synthesized and their spectroscopic properties evaluated. Halogenation ortho or para to the bridging oxygen in the BAPTA nucleus had a more pronounced weakening effect on binding affinity than in the meta position in the fura derivatives. Two new excitation ratioable fluorescent calcium indicators, benzothiaza-1 and 2, were also synthesized. Kd values of 400 nM to 5.3 μM [Ca2+] were observed in these families of new probes.
