Author/Authors :
James E. Sheppeck II، نويسنده , , Heidi Kar، نويسنده , , Luke Gosink، نويسنده , , Jeffrey B. Wheatley، نويسنده , , Erik Gjerstad، نويسنده , , Siobhan M. Loftus، نويسنده , , Alexi R. Zubiria، نويسنده , , James W. Janc، نويسنده ,
Abstract :
A statistically exhaustive, 8800 compound tripeptidal amidomethylcoumarin library was synthesized as discreet compounds using solid-phase combinatorial chemistry. A subset of the compounds was purified by HPLC and tested in a high-throughput fluorometric assay against several known serine and cysteine proteases to demonstrate the utility of this library for profiling protease substrate specificity.
