Title of article :
Synthesis and use of FSCPX, an irreversible adenosine A1 antagonist, as a ‘Receptor Knock-Down’ tool
Author/Authors :
Jacqueline E. van Muijlwijk-Koezen، نويسنده , , Henk Timmerman، نويسنده , , Richard P. van der Sluis، نويسنده , , Andrea C. van de Stolpe، نويسنده , , Wiro M. P. B. Menge، نويسنده , , Margot W. Beukers، نويسنده , , Piet H. van der Graaf، نويسنده , , Miriam de Groote، نويسنده , , Adriaan P. Ijzerman، نويسنده ,
Abstract :
A new preparative synthetic route for the irreversible adenosine A1 antagonist 8-cyclopentyl-3-N-[3-((3-(4-fluorosulphonyl)benzoyl)-oxy)-propyl]-1-N-propyl-xanthine (FSCPX, 1) is described. The availability of ample amounts of the irreversible antagonist FSCPX allowed us to use FSCPX as a research tool for adenosine A1 receptors in in vivo experiments. After verification of the irreversible antagonistic function of FSCPX in in vitro experiments, FSCPX was used successfully as a ‘receptor knock-down’ tool in in vivo experiments on conscious rats.
