Coupling of the antennapedia third helix to a potent antagonist of the p53/hdm2 protein–protein interaction

The use of cell-membrane translocating sequences for intracellular delivery of peptides can be a powerful approach to validate drug discovery targets in cellular settings. To accomplish this, a protocol has been implemented to couple the antennapedia third helix (residues 43–58) to a potent antagonist of the p53/hdm2 protein–protein interaction without affecting its in vitro inhibitory activity.