Author/Authors :
Nadia J. Kershaw، نويسنده , , Mridul Mukherji، نويسنده , , Colin H. MacKinnon، نويسنده , , Timothy D. W. Claridge، نويسنده , , Barbara Odell، نويسنده , , Anthony S. Wierzbicki DPhil، نويسنده , , Matthew D. Lloyd، نويسنده , , Andrea G. Prescott and Christopher J. Schofield، نويسنده ,
Abstract :
Phytanoyl-CoA 2-hydroxylase (PAHX), an iron(II) and 2-oxoglutarate-dependent oxygenase, catalyses an essential step in the mammalian metabolism of β-methylated fatty acids. Phytanoyl-CoA was synthesised and used to develop in vitro assays for PAHX. The product of the reaction was confirmed as 2-hydroxyphytanoyl-CoA by NMR and mass spectrometric analyses. In accord with in vivo analyses, hydroxylation of both 3R and 3S epimers of the substrate was catalysed by PAHX. Both pro- and mature- forms of PAHX were fully active.
