Hydrolysis of linear DNA duplex catalyzed by Co(III) complex of cyclen attached to polystyrene

To design artificial restriction enzymes, synthetic catalytic centers that effectively hydrolyze linear double-stranded polydeoxyribonucleotides are needed. The Co(III) complex of cyclen (CoCyc) attached to polystyrene derivatives hydrolyzes linearized pUC18 DNA with half-lives as short as 30 min at 25 °C. The catalytic activity of CoCyc is enhanced by >150 times on attachment to the resin.