Biotinylated lithocholic acids for affinity chromatography of mammalian DNA polymerases α and β

Biotinylated lithocholic acids have been synthesized. The compounds inhibited mammalian DNA polymerases α and β with dose-dependent manner. The streptavidine columns conjugated with the synthetic biotinylated compounds chromatographed both two enzymes eluted by KCl solution at the different concentrations.