Absorption Studies on Aminoglycoside Binding to the Packaging Region of Human Immunodeficiency Virus Type-1

The binding of paromomycin, neomycin B, and three analogues of neomycin to a 176-mer RNA from the packaging region of HIV-1 (LAI) has been studied using UV absorption spectroscopy at wavelengths between 200 and 300 nm. From plots of absorption as a function of drug concentration, values of binding constants for these drugs on RNA were determined.