Chemical synthesis and calcium release activity of N1-ether strand substituted cADPR mimic

8-Chloro cyclic inosine 5′-diphosphate ethoxymethyl ether 3 was synthesized by means of chemical method from protected inosine via phenylthio-type biphosphate substrate. The detection of Ca2+ release activity shows that 3 is a potent agonist of cADPR and has activity in intact Hela cells.