Syntheses of dendritic linkers containing chlorambucil residues for the preparation of antibody–multidrug immunoconjugates

A novel dendritic molecule with nine chlorambucil (CBL) residues on the surface and a maleimide moiety at the core terminus was synthesized using a convergent synthetic methodology. This molecule is ready for attachment to single-chain Fv antibodies (scFvs) to form antibody–multidrug immunoconjugates in an effort to study the relevance of drug/antibody molar ratio and the potency of these drug–antibody immunoconjugates. A monomer and a trimer with a similar structural motif were also prepared for comparative purposes.