A novel solid support for synthesis of oligonucleotide 3′-phosphorothioate monoesters

A new reagent immobilized on solid support allowing for solid-phase synthesis of oligonucleotides with a 3′-terminal phosphorothioate monoester is described. The support is compatible with phosphoramidite chemistry for automated oligonucleotide synthesis. Final deprotection with ammonia under standard conditions leads to oligonucleotide 3′-terminal phosphorothioate.