Title of article
A low background high-Throughput screening (HTS) fluorescence assay for lipases and esterases using acyloxymethylethers of umbelliferone
Author/Authors
Emmanuel Leroy، نويسنده , , Nicolas Bensel، نويسنده , , Jean-Louis Reymond، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2003
Pages
4
From page
2105
To page
2108
Abstract
Esters and acyloxymethyl ethers of umbelliferone were evaluated as fluorogenic substrates for lipases and esterases with respect to stability and resistance to non-specific hydrolysis. Isobutyryloxymethyl ether 1c and pivaloxymethyl ether 1d were found to be optimal substrates for enzyme assays, particularly with respect to HTS applications.
Journal title
Bioorganic & Medicinal Chemistry Letters
Serial Year
2003
Journal title
Bioorganic & Medicinal Chemistry Letters
Record number
793306
Link To Document