Glycosynthase-Catalysed syntheses at pH below neutrality

The use of the new glycosynthase Ta-β-GlyE386G and of the already known Ss-β-GlyE387G for the synthesis of interesting 4-methylumbelliferyl disaccharides and for the galactosylation of α- and β-xylosides of 4-penten-1-ol is reported. The results show satisfactory yields of reaction in presence of low excesses of acceptors and demonstrated that the high activity of these enzymes at pH below neutrality is applicable in the transfer of glucose as well as of galactose from the preferred 2-NP-based donors.