Regeneration and Histological of Plants Derived from Leaf explants in vitro Culture of Strawberry

ABSTRACT: In the current work attempts were made to investigate culture of different explants derived from in vitro seedlings of Fragaria species. Effects of explants on regeneration response of strawberry were examined based on four parameters, include number of shoot per explant, shoot length per explants, number of root per explants and percentage of rooting. The best response for callus induction was observed for leaf explants was obtained on MS supplemented with BAP 2 mg/l and NAA 0.5 mg/l. After 4 weeks, the highest callus growth transferred in same medium callus induction for direct regeneration that was BAP 2 mg/l and NAA 0.5 mg/l. The highest efficiency and high frequency of shoot formation occurred after 30 days. Elongation of shoot buds was obtained on MS medium containing 2 mg/l BAP + 0.1 mg/l NAA. Regenerated shoots rooted best on the same medium of elongation. The regenerated plantlets have been successfully established in pit mass soil. Subsequently, they were shifted to the field conditions. Regenerated plantlets resemble the parent plants morphologically and cytological. Histological study of the cultures showed the presence of meristemoids, somatic embryos and embryoids. We concluded that the present protocol can be efficiently used for mass propagation of the strawberry.