Study Effect of IBA Hormone and Shoot Tip Length Levels on Rooting of Poinsettia under In-Vitro Condition

ABSTRACT: In order to study the effects of indole-3-butyric acid (IBA) hormone levels and shoot tip length levels in vitro culture of poinsettia (Euphorbia pulcherrima wild), an experiment as factorial in RCBD with four replications conducted during 2010 at Research Laboratory of Faculty of Agriculture, Lahijan University in Iran. Factors were IBA hormone levels (A1= 0. 1, A2= 0.5, A3= 1 and A4= 3 mg/L), and shoot tip length levels (S1=10mm and S2=20mm). The culture beds were contained MS, sucrose (3%) and agar (75%). The results show Interaction effect of IBA hormone and shoot tip length levels on percent of rooting; root number and root length in vitro culture of poinsettia had a significant difference in 1%. Comparison of mean between interaction IBA hormone and shoot tip length levels show that, percent of rooting (100 %), and root length (17.89 mm) were obtained from A4S2 treatment and the highest root number (5.13) was obtained from A3S2 treatment.