Metalated hybrid polymers as catalytic reagents for phosphate ester hydrolysis and plasmid modification

Pendant pyrazolylcyclophosphazene containing hybrid cross-linked polymer (CPPL) has been utilized for binding Zn(II). The metalated polymer (CPPL–Zn) has been found to be very effective catalyst for the hydrolysis of a RNA model phosphodiester substrate [2-(hydroxypropyl)-p-nitrophenyl phosphate, hNPP]. In addition, CPPL–Zn also cleaved supercoiled plasmid DNA pBR322 thus providing a novel structural motif of inorganic–organic hybrid polymers as synthetic nucleases.