Title of article
Preparation of highly active α-chymotrypsin for catalysis in organic media
Author/Authors
Ipsita Roy، نويسنده , , Munishwar N Gupta، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2004
Pages
3
From page
2191
To page
2193
Abstract
A simple one step process for the preparation of free α-chymotrypsin, using an organic solvent to precipitate the enzyme from a buffered solution, followed by washing with organic solvents, is described. This preparation gave 132 times greater esterification activity than lyophilized powder.
Keywords
amino acids , enzymes , peptides , catalysis , Transesterification.
Journal title
Bioorganic & Medicinal Chemistry Letters
Serial Year
2004
Journal title
Bioorganic & Medicinal Chemistry Letters
Record number
794365
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