Title of article :
Preparation of highly active α-chymotrypsin for catalysis in organic media
Author/Authors :
Ipsita Roy، نويسنده , , Munishwar N Gupta، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Abstract :
A simple one step process for the preparation of free α-chymotrypsin, using an organic solvent to precipitate the enzyme from a buffered solution, followed by washing with organic solvents, is described. This preparation gave 132 times greater esterification activity than lyophilized powder.
Keywords :
amino acids , enzymes , peptides , catalysis , Transesterification.
Journal title :
Bioorganic & Medicinal Chemistry Letters
Journal title :
Bioorganic & Medicinal Chemistry Letters
