Synthesis of novel fluorescent-labelled dinucleoside polyphosphates

A novel tandem synthetic–biosynthetic procedure is described for the synthesis of four new fluorescent dinucleoside polyphosphates: mant–Ap4A, mant–AppCH2ppA, TNP–Ap4A and TNP–AppCH2ppA. These compounds are expected to supplement the existing etheno (ε) and 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) labelled derivatives, being the fluorescent probes of choice to investigate polyphosphate/enzyme binding behaviour.