Effective inhibition of HIV-1 replication in cultured cells by external guide sequences and ribonuclease P

We examined the suppressive effect of HIV-1 RNA gene cleavge on HIV-1 expression, using the catalytic RNA subunit RNase P and the 3′-half tRNATry [external guide sequence (EGS)] in cultured cells. HIV-1 expression was inhibited by the tRNAmet-EGS-U5 and U6-EGS-U5 from the tRNAmet and U6 promoters, respectively. There was no difference in the inhibitory effects on HIV-1 expression between the tRNAmet and U6 promoters.