Title of article
Proposal of new modification technique for linear double-stranded DNAs using the polysaccharide schizopyllan
Author/Authors
Takahisa Anada، نويسنده , , Hideshi Matsunaga، نويسنده , , Ryouji Karinaga، نويسنده , , Kazuya Koumoto، نويسنده , , Masami Mizu، نويسنده , , Koji Nakano، نويسنده , , Seiji Shinkai، نويسنده , , Kazuo Sakurai، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2004
Pages
5
From page
5655
To page
5659
Abstract
A natural polysaccharide schizophyllan (SPG) has been known to form a stable complex with poly(dA). We attached a poly(dA)80 tail to the both ends of a linear double-stranded DNA, which had been prepared from a plasmid DNA vector. The poly(dA) tailed DNA verified to form complex with SPG by gel electrophoresis and atomic force microscopy (AFM). AFM images indicated that the complexes exhibit a dumbbell-like architecture, that is, quite similar to that of adenovirus genome. The complex demonstrated excellent exonuclease resistance, probably because of the protection effect by SPG complexation.
Keywords
Polysaccharide/DNA complex , AFM.qThis paper is series of polysaccharide–polynucleotide complex (36).* Corresponding author. Tel.: +81 93 695 3298 , fax: +81 93 6953390 , e-mail: sakurai@env.kitakyu-u.ac.jp , Schizopyllan
Journal title
Bioorganic & Medicinal Chemistry Letters
Serial Year
2004
Journal title
Bioorganic & Medicinal Chemistry Letters
Record number
795042
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