Title of article :
Efficient cleavage of RNA, enhanced cellular uptake, and controlled intracellular localization of conjugate DNAzymes
Author/Authors :
Takanori Kubo، نويسنده , , Kengo Takamori، نويسنده , , Kenichi Kanno، نويسنده , , Bakalova Rumiana، نويسنده , , Hideki Ohba، نويسنده , , Miho Matsukisono، نويسنده , , Yasuo Akebiyama، نويسنده , , Masayuki Fujii، نويسنده ,
Abstract :
Conjugate DNAzymes with polyamines and peptides were successfully prepared by solid phase fragment condensation (SPFC) and showed up to 4.2 times higher catalytic efficiency (kcat/Km) and enhanced tolerance against DNase 1digestion. To be pointed out, intracellular localization of DNAzymes could be controlled by conjugated with naturally occurring signal peptides responsible for nuclear cytoplasmic transport of proteins.
