Title of article
Detection of aptamer–protein interactions using QCM and electrochemical indicator methods
Author/Authors
Tibor Hianik، نويسنده , , Veronika Ostatn?، نويسنده , , Zuzana Zajacov?، نويسنده , , Ekaterina Stoikova، نويسنده , , Gennady Evtugyn، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2005
Pages
5
From page
291
To page
295
Abstract
We report novel method of detection thrombin–aptamer interaction based on measurement the charge consumption from the electrode covered by DNA aptamers to an electrochemical indicator methylene blue (MB), that is bounded to a thrombin. The binding of thrombin to an aptamers has been detected also by QCM method in flow measuring cell. We showed that using MB it is possible to detect thrombin with high sensitivity and selectivity.
Keywords
Aptamer , methylene blue , Cyclic voltammetry , Charge consumption , thrombin
Journal title
Bioorganic & Medicinal Chemistry Letters
Serial Year
2005
Journal title
Bioorganic & Medicinal Chemistry Letters
Record number
795195
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