Title of article
Discovery of selective aldo-keto reductase ligands—an on-bead assay strategy
Author/Authors
Seth Dixon، نويسنده , , Lori Robins، نويسنده , , Robert A. Elling and David K. Wilson، نويسنده , , Ruiwu Liu، نويسنده , , Kit S. Lam، نويسنده , , David K. Wilson، نويسنده , , Mark J. Kurth، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2005
Pages
5
From page
2938
To page
2942
Abstract
An enzyme labeling and screening strategy for the discovery of ligands selective in binding two structurally similar members of the aldo-keto reductase family of enzymes is reported. The resulting fluorescence microscope data obtained by screening a 74,088 member library led to the identification of selective ligands for aldose reductase (ALR2) and aldehyde reductase (ALR1). Resynthesis results validate the selectivity of these ligands.
Keywords
Selective binding , Protein fluorescent labeling , Protein binding , ALR1 , ALR2 , On-bead assay
Journal title
Bioorganic & Medicinal Chemistry Letters
Serial Year
2005
Journal title
Bioorganic & Medicinal Chemistry Letters
Record number
795703
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