Title of article
Chemically modified oligonucleotides with efficient RNase H response
Author/Authors
Birte Vester، نويسنده , , Anne Marie Boel، نويسنده , , Sune Lobedanz، نويسنده , , B. Ravindra Babu، نويسنده , , Michael Raunkj?r، نويسنده , , Dorthe Lindegaard، نويسنده , , Raunak، نويسنده , , Patrick J. Hrdlicka، نويسنده , , Torben H?jland، نويسنده , , Pawan K. Sharma، نويسنده , , Surender Kumar Pal، نويسنده , , Poul Nielsen، نويسنده , , Jesper Wengel، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2008
Pages
5
From page
2296
To page
2300
Abstract
Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3–ON12 and ON15–ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.
Keywords
Modified oligonucleotide , RNase H cleavage , antisense oligonucleotide , Antisense technology , Modified nucleotide
Journal title
Bioorganic & Medicinal Chemistry Letters
Serial Year
2008
Journal title
Bioorganic & Medicinal Chemistry Letters
Record number
799343
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