Binding properties of positively charged deoxynucleic guanidine (DNG), AgTgAgTgAgT and DNG/DNA chimeras to DNA

The melting properties of hexameric oligonucleotide AgTgAgTgAgT, in which the phosphodiester linkages of the DNA have been replaced by guanidium linkages, have been evaluated. Using the juvenile esterase gene as a target, the binding of a 20-mer DNG/DNA chimera that includes AgTgAgTgAgT is more than 105.7 stronger than the binding of 20-mer composed solely of DNA.