Title of article :
Simultaneous identification of multiple receptors of natural product using an optimized cDNA phage display cloning
Author/Authors :
Qing-Li He، نويسنده , , Hui Jiang، نويسنده , , Feng Zhang، نويسنده , , Hai-Bao Chen، نويسنده , , Gong-Li Tang، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2008
Pages :
4
From page :
3995
To page :
3998
Abstract :
Simultaneously isolating more than one receptor of natural product remains a challenge to chemical genetics. Using cyclosporine A as an affinity probe and an optimized phage display cloning procedure, not only cyclophilin A, but also cyclophilin B was isolated as the full-length gene clone from a human brain cDNA library. This optimized protocol can be used to select protein targets of chemicals dependent on the binding affinity rather than on the relative abundance in cells.
Keywords :
Cyclosporine A , Display cloning , Cyclophilin A , Cyclophilin B
Journal title :
Bioorganic & Medicinal Chemistry Letters
Serial Year :
2008
Journal title :
Bioorganic & Medicinal Chemistry Letters
Record number :
799701
Link To Document :
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