Title of article
NaCl improves siRNA delivery mediated by nanoparticles of hydroxyethylated cationic cholesterol with amido-linker
Author/Authors
Yoshiyuki Hattori، نويسنده , , Ayako Hagiwara، نويسنده , , Wuxiao Ding، نويسنده , , Yoshie Maitani، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2008
Pages
5
From page
5228
To page
5232
Abstract
We investigated the effect of amido- (NP-OH) and carbamate linkers (NP-HAPC) in nanoparticles composed of hydroxyethylated cationic cholesterol on siRNA transfection. The presence of NaCl in forming a NP-OH nanoplex increased the suppressive effect of gene expression by increasing the size of the nanoplex and changing the cellular uptake mechanism from membrane fusion and clathrin-mediated endocytosis to clathrin- and caveolae-mediated endocytosis.
Keywords
Synthetic siRNA , siRNA delivery , Nanoplex , PC-3 cells , Caveolae-mediated endocytosis , Membrane fusion , Cationic nanoparticles , Transfection
Journal title
Bioorganic & Medicinal Chemistry Letters
Serial Year
2008
Journal title
Bioorganic & Medicinal Chemistry Letters
Record number
799986
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