Effect of BA and GA3 Application on Poinsettia (Euphorbia pulcherrima Willd) under In-Vitro Conditions

ABSTRACT: In order to study the effects of benzyladenine (BA) and giberlin (GA3) hormone management in vitro culture of poinsettia (Euphorbia pulcherrima wild), an experiment as factorial in RCBD with three replications was conducted during 2010 at Research Laboratory of Faculty of Agriculture, Lahijan Branch, Islamic Azad Lahijan University in Iran. Factors were GA3 hormone levels (G1= 0, G2= 1 and G3= 5 mg/L), and BA hormone levels (B1=0.01, B2=0.1, B3=1 and B4=5 mg/L). The culture bed was contained MS, sucrose (3%) and agar (75%). The results showed that Interaction effect of BA and GA3 levels on shoot length and leaf number in had a significant difference in 1% probability level. Mean comparison between interaction effect of BA and GA3 levels showed that, the highest shoot length of poinsettia was obtained from G3B2 (21.73 mm) and G3B3 (22.60 mm) treatments, and the highest leaf number of poinsettia was obtained from G1B3 treatment (3.80).