Proline Accumulation, Protein Pattern and Photosynthesis in Bacopa Monniera Regenerants Grown under NaCl Stress

In the leafstem moss Mnium affine two superoxide dismutase (SOD) isoforms were found in chloroplasts and two in mitochondria. Four other isozymes were probably cytosolic and two of them had high activity and thermostability and were very sensitive to H2O2. On the other hand, one of the mitochondrial isoenzymes was very sensitive to high temperature. The activity and thermosensitivity of SOD was considerably dependent on calcium and zinc ions. The effect was different for the individual isoforms and related to their subcellular distribution. Calcium ions predominantly activated and stabilized one cytosolic and the mitochondrial SODs, while zinc ions influenced one chloroplastic and two cytosolic SODs.