Promotion of Remyelination by Adipose Mesenchymal Stem Cell Transplantation in A Cuprizone Model of Multiple Sclerosis

?????????????????????? Multiple sclerosis (MS) is an immune-mediated demyelinating disease of the central nervous system (CNS). Stem cell transplantation is a new therapeutic approach for demyelinating diseases such as MS which may promote remyelination. In this study, we evaluate the remyelinating potential of adipose mesenchymal stem cells (ADSCs) and their effect on neural cell composition in the corpus callosum in an experimental model of MS. ???????????????????????????????????????????? This experimental study used adult male C57BL/6 mice. Cul- ????????????????????????????????????????????????????????????????????????+,CD90+, CD31-,CD45-, and labeled by PKH26. Animals were fed with 0.2% w/w cuprizone added to ground breeder chow ad libitum for six weeks. At day 0 after cuprizone removal, mice were randomly divided into two groups: the ADSCs-transplanted group and the control vehicle group (received medium alone). Some mice of the same age were fed with their normal diet to serve as healthy control ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? ????????????????????????????????- scope. At ten days after transplantation, the mice were euthanized and their cells analyzed ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? Results were analyzed by one-way analysis of variance (ANOVA). ?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? ??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????- uation revealed enhanced remyelination in the transplanted group compared to the control vehicle group. Flow cytometry analysis showedan increase in Olig2 and O4 cells and a decrease in GFAP and Iba-1 cells in the transplanted group. ????????????????????????Our results indicate that ADSCs may provide a feasible, practical way for remyelination in diseases such as MS.