Evaluation of an indirect enzyme-linked immunoassay for presumptive serodiagnosis of Brucella ovis in sheep

An indirect enzyme-linked immunoassay (IELISA) for the detection of antibodies to Brucella ovis was evaluated. Relative to the complement fixation test (CFT) the sensitivity of the IELISA was 96.3% and the specificity was 99.6%. The sensitivity and specificity obtained in this study were comparable to ELISAs and CFTs of other studies in which B. ovis isolation was used for evaluation making it a choice to replace current serological tests such as the agar gel immunodiffusion test and the CFT. © 2003 Elsevier Science B.V. All rights reserved