A multistage system of microbial fed-batch fermentation and its parameter identification Original Research Article

In fed-batch fermentation of glycerol bio-dissimilation to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae, glycerol and alkali are fed into the reactor to provide nutrient and maintain a suitable environment for cells growth. Taking the feeding process as a time-continuous process, we present a nonlinear multistage dynamical system to formulate the fed-batch fermentation. In view of the big errors between experimental data and computational values, a parameter identification model is established. Finally, an improved Nelder–Mead simplex search method is developed to seek the unknown parameters in the model. Numerical simulations show that the multistage dynamical system can describe the fed-batch process better, compared with the previous results.