Phytochemical analysis and Cytotoxicity studies of Tylophoraindica leaves in BHK-21 cells

Tylophoraindicais an annual or perennial medicinal plant which is used in several traditional medicines to cure various diseases. Tylophoraindica has increasingly popular for treatment of asthma. These weeds have been known to possess immunomodulatory, hypoglycaemic, antiallergicand antimicrobial properties However, its anticancer properties are not well known; therefore, it had been planned to study its anticancer properties in BHK-21 cells. Acetone, ether and aqueous extracts of the leaves of Tylophoraindica were screened for their anticancer properties. The cells were seeded with all the extracts separately and then allowed to grow for 24hrs; the cell growth was inhibited within 24hrs. The cytopathology observed were included rounding and clumping of cells, detachment of cells, flagging of cells and apoptosis. Acetone and ether extracts showed better response than that of its aqueous extract. The concentration of 10 mg/ml of acetone extract inhibited the cancerous cell growth.