Isozyme and RAPD Markers in Relation to In Vitro Morphogenesis and In Vivo Development of Vigna radiata (L.) Wilczek.

Vigna radiata (L.) Wilczek, popularly known as “mung bean,” is an important protein supplement in vegetarian diets in many countries of Asia. It has a short life cycle (55–70 days) and fits well into many cropping systems, including rice and sugarcane, under rain-fed and irrigated conditions. The present review deals with the data available on plant regeneration of this species. Both shoot-tip multiplication and somatic embryogenesis have been compared on the basis of retrospective as well as recent reports. Molecular markers, especially random amplified polymorphic DNA (RAPD), have been used to compare results obtained from in vitro and in vivo studies on various species of Vigna . Isozyme markers such as esterase and superoxide dismutase, which are expressed during in vitro regeneration and in vivo development of Vignaspecies, are also included in this article.