Author/Authors :
Sadek ، KM نويسنده Department of Biochemistry, Faculty of Veterinary Medicine, Edfina, Alexandria University , , Taha ، NM نويسنده Department of Biochemistry, Faculty of Veterinary Medicine, Edfina, Alexandria University , , Korshom ، M نويسنده Department of Biochemistry, Faculty of Veterinary Medicine, Edfina, Alexandria University , , Mandour ، A نويسنده Department of Biochemistry, Faculty of Veterinary Medicine, Edfina, Alexandria University ,
Abstract :
Alanine aminotransferase (ALT) is one of special liver enzymes and used as indicator of liver damage. ALT was purified from the liver of rabbits treated with Acrylamide (ACR) with or without garlic. The results revealed that, Acrylamide is a non competitive inhibitor of ALT. In the contrary, garlic increases the affinity of ALT to its substrate. The results showed also that, the optimal temperature, pH and incubation time was 37 ?C, 7.5 and 30 minutes respectively.
