A Study on the Effect of Different Concentration of Plant Hormones (BAP, NAA, 2, 4-D, and Kinetin) on Callus Induction in Brassica Napus

ABSTRACT: In the current study, an efficient medium for callus production in Brassica napus genotype SLM -046 was established. Three media complemented with different plant hormones were investigated. The based medium was MS (Murashige&Skoog, 1962) supplemented with 30 gl-1 sucrose and 0.8 % agar. Seeds of B. napus after surface sterilization were placed on germination medium. Hypocotyl explants from 5-7-day-old seedling were cut and transferred to the following media: a) MS complemented with BAP, NAA, and 2,4-D hormones, b) MS plus NAA and kinetin, c) MS with only 2,4-D. Then, every 2 weeks the explants were subcultured on the same medium. To determine the effect of these media, an experimental design, namely Completely Randomized Design (CRD), with 3 replications was used. Number, size, and weight of calli were measured as desirable traits after 4 weeks. The statistical analysis showed that there were significant differences between these media. The highest callus production was obtained from 5-day-old seedling in first medium. Thus, the results showed that interactions between BAP, NAA, and 2,4-D hormones were the most effective factor for callus induction and production. The lowest amount of callus was just from medium with 2,4-D.