The Effect of Leptin in Increasing the Quality of Goat Oocyte Maturation in Vitro

The aim of the research was to determine the effects of leptin in accelerating oocytes maturation. In this research, the addition of leptin in oocyte maturation medium to tissue culture medium-199 (TCM-199) + 10% fetal bovine serum (FBS) at concentrations of 0, 50, 100, 250, 500, 750 and 1000 ng/ml in incubation time 18, 22, 26 and 30 hours significantly increased oocytes percentage up to metaphase II (M II) along with increasing incubation time. The concentration of750 ng/ml with an incubation time of 30 hours is the most optimal in endorsing oocytes to reach M II.