Author/Authors :
Wang ، Z. نويسنده College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. , , Huang ، J. نويسنده Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China. , , Xia ، P. نويسنده College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. , , Song ، L. نويسنده Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China. , , Ju، Z. نويسنده , , Li ، Q. نويسنده Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China. , , Wang ، C. نويسنده Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China. , , Zhong، Zhuang J. نويسنده , , Wang ، G. نويسنده College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. ,
Abstract :
The 1,369bp expressed clone sequence of clusterin (CLU) cDNA was used to evaluate transcriptional changes in the mammary tissue in Chinese Holstein Cattle. Two transcript variants (X-CLU and Y-CLU) were discovered in mastitis mammary tissue. X-CLU is the main transcript and Y-CLU is the splice transcript produced by the alternative 3ʹ and 5ʹ splice sites. Real-time PCR analysis confirmed the increased expression of X-CLU mRNA in mastitis mammary tissue (P < 0.05). Western blot analysis showed that the clusterin gene in the Chinese Holstein cows had two distinct bands of about 60 kDa and 80 kDa in both normal and mastitis mammary tissues.
