Author/Authors :
Xia ، P. نويسنده College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. , , Wang ، Z. نويسنده College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. , , Wang ، F. نويسنده College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. , , Wang ، C. نويسنده Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China. , , Huang ، J. نويسنده Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China. , , Li، Q. نويسنده Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China. , , Wang ، G. نويسنده College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. ,
Abstract :
Heat shock factor1 (HSF1) plays a key role in the regulation of the transcription of both well-known HS genes and other numeral genes and also is treated as a regulator even in the cancer and life span. In our study, novel SNP and isoform of mRNA following by 54bp downstream have been found in transactivation domain (TAD) of HSF1 gene in Chinese Holstein cattle. Firstly, it has been demonstrated that novel SNP was found to be associated with mastitis. Secondly, new isoform, HSF1b (24bp bases of exon skipping and following +1bp base splice-site mutation in the downstream of +141bp bases), has been discovered. Finally, the result also showed that the splicing of new isoform occurs only in the tissues with TT and CT genotypes or only in the plasmids with T allele. Above all, the discovery of new SNP and RNA isoform revealed the SNP was much important in breeding selection to prevent from the mastitis and supported a new way to study whether T allele could possibly regulate the splicing of HSF1b.
