Entomotoxicity assay of Nano-particle 3-(Zinc oxide ZnO) Against Sitophilusoryzae Under Laboratory and Store Conditions in Egypt

ABSTRACT: when nano, zinc oxideZnO were tested against Sitophilusoryzae,the number of mortality of S. oryzaewere significantly increased to 49.4±4.4, 57.8±5.3 and 50.6±3.2 individuals after treated with 3% of ZnO after 7, 21and 45 days, as compared to 1.0 ± 2.8, 1.0 ± 5.1 and 2.0 ± 3.1, respectively in the control. When Sitophilusoryzae were treated with ZnO under laboratory conditions, the number of mortality scored a higher mortality reached to 37.1±3.2, 40.4±3.4 and 44.7±5.1 individuals after treated with1% of ZnO nanoparticles as compared to 2.0 ±3.8, 1.0 ±5.1 and 2.0 ±3.1 individualsIn 0.2% treatments with nanoZnO, the number mortality of S. oryzae were significantly increased to 14.1±2.3, 28.2±8.8 and 29.8±3.9 individuals after nanoZnO treatments as compared individuals in the control Table 2. At higher concentrations of 3% the mortality percent were significantly increased to 89.0±1.1 individuals after 45 days of applications as compared to 3.0±2.6 individuals in the control,The number of eggs laid /female were significantly reduced. After 100 and 120 days the number of eggs laid/ female were significantly decreased to 6±1.0 and 11±0.51 as compared to 99.1±1.43 and 97.2±1.82 in the control Table 4. day The % reduction values in the number of laid eggs and adult emergence after 120 days were 91 and 90%, respectively in case of Zno application on foam covering gunny bags provided promising oviposition deterrence, toxicity and suppressing S.oryzae infestation, persistence and protecting rice seeds from beetles’ infestation for 120 days during storage.