A novel enzymic determination of maltobionate

A novel enzymic determination of maltobionate with three enzymes (a new enzyme, maltobionate α-d-glucohydrolase [EC 3.2.1.-], gluconate kinase [EC 2.7.1.12], and 6-phosphogluconic dehydrogenase [EC 1.1.1.44]) is described. It fulfilled the need for an accurate, specific, and rapid assay of maltobionate, as shown by the procedure, the linearity of calibration curve, and the analytical precision, and was simple as compared to an HPLC approach. There was a good correlation (r = 0.998) between the results obtained by the enzymic and HPLC methods. As an application, the concentrations of maltobionate in the culture broth of Pseudomonas taetrolens IFO 3460 were determined by the proposed method, affording the maltobionate production from maltose in the course of cultivation of this strain.