Author/Authors :
Haralambos Parolis، نويسنده , , Lesley A.S. Parolis، نويسنده , , Isabel F. Bo?n، نويسنده , , Francisco Rodr?guez-Valera، نويسنده , , G?ran Widmalm، نويسنده , , Maria Cristina Manca، نويسنده , , Per-Erik Jansson، نويسنده , , Ian W. Sutherland، نويسنده ,
Abstract :
The halophilic Archaeon Haloferax mediterranei exudes into the growth medium a high molecular weight sulfated polysaccharide. The structure of the repeating unit of this polymer was determined by a combination of glycose, methylation, and sulfate analysis, periodate oxidation, and 1D and 2D NMR spectroscopic analysis of the native and periodate-oxidised/reduced polysaccharides. The location of the sulfate group was established from the 1H and 13C NMR data. The structure of the repeating unit of the polysaccharide may be written as →4)-β-D-GlcpNAcA-(1→6)-α-D-Manp-(1→4)-β-D-GlcpNAcA-3-O-SO3-(1 →
Keywords :
Exopolysaccharide , Haloferax mediterranei , NMR spectroscopy , Sulfate , Periodate oxidation
